LSM 780 NLO (C3.243)

Microscope

Inverted Zeiss LSM 780

2016-01-28 08.55.18

Lasers

  • 1-photon
    • diode 405 (DAPI)
    • Argon 458, 488, 514 (CFP, GFP, YFP)
    • HeNe 543 (RFP)
    • HeNe 633 (Far red)
  • 2-photon
    • Chameleon Vision II (690-1064 nm) (Dapi, CFP, GFP, YFP, RFP)

Other light sources

  • HALOGEN for brightfield transmitted light
  • X-Cite series 120PC for epifluorescence (blue, green, red long pass emission) The fluorescent illuminator is equipped with neutral density filter for intensity modulation.

Objectives (See Objective Guide)

  • EC Plan Neofluar 5x/0.16 M27 (WD 18 500 um)
  • EC Plan Neofluar 10x/0.30 M27 (WD 5200 um)
  • Plan Apochromat 20x/0.8 M27 (WD 550 um)
  • LD C Apochromat 40x/1.1 W Korr M27 (WD 620 um)
  • LD LCI Plan Apochromat 25x/0.8 Imm Korr DIC M27 (WD 570 um)
  • (alpha Plan-Fluar 100x/1.45 oil; available but not in position)

Software

ZEN 2012 SP1 (64-bit) release 8.1
Extra modules: TILES, PHYSIOLOGY

Filters


Detectors

  • internal PMT 1
  • internal GaAsP detector array (32x)(spectral imaging possible)
  • internal PMT 2
  • T-PMT (transmitted light)

Also available

  • Motorized stage
  • Incubator
  • CO2 control

Documents:

  • start up (Link) and shut down (Link) procedures